Antibiotic resistance of bacterial biofilms limits available treatment methods for implant-associated orthopaedic infections. This study evaluated the effects of applying cathodic voltage-controlled electrical stimulations (CVCES) of 1.5 V and 1.8 V (vs. Ag/AgCl) to coupons of commercially pure titanium (cpTi) incubated in cultures of methicillin-resistant Staphylococcus aureus (MRSA) and Acinetobacter baumannii (A. baumannii) as a method of preventing bacterial attachment. Stimulations were applied for 2, 4, and 8 h and coupon-associated and planktonic colony-forming units (CFU) were enumerated following stimulation. Compared to open circuit potential (OCP) controls, CVCES for 4 h at 1.8 V significantly reduced 4 7 coupon-associated MRSA CFU by 99.9% (1.30 107 vs. 4.45 10 , p = 0.047) and A. baumannii coupon4 associated CFU by 99.9% (1.64 10 vs. 5.93 10 , p = 0.001) and reduced planktonic CFU below detectable levels for both strains. CVCES at 1.8 V for 8 h also reduced coupon-associated and planktonic CFU below detectable levels for each strain. CVCES at 1.5 V for 4 and 8 h, and 1.8 V for 2 h did not result in clinically relevant reductions. For 4 and 8 h stimulations, the current density was significantly higher for 1.8 V than 1.5 V, an effect directly related to the rate of water and oxygen reduction on the cpTi surface. This significantly increased the pH, a suspected influence in decreased CFU viability. The voltagedependent electrochemical properties of cpTi likely contribute to the observed antimicrobial effects of CVCES. This study revealed that CVCES of titanium could prevent coupon-associated and planktonic CFU of Gram-positive MRSA and Gram-negative A. baumannii from reaching detectable levels in a magnitude-dependent and time-dependent manner.